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KMID : 0363219770150010027
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Korean Journal of Dermatology
1977 Volume.15 No. 1 p.27 ~ p.30
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New Plexiglass Hanging Drop Culture Apparatus
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Abstract
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The purpose of this paper is to describe a new apparatus which utilizes the principle of Van Tieghem´s hanging drop culture method. After using various methods of culturing fungi authors have found van Tieghem´s method to yield the most satifsying result. Until now, this method has been slightly more tedious due to durability, storage, and quantity of culture necessary for each species.
The new apparatus consists of 20mm plexiglass tubing cut in 16mm lengths and attache to a 16cm x 12cm x 2mm plexiglass plate with paraffin or manicure. These tubes are ranged in rows approximately 6cm apart with at least 8mm between tubes. Plates of varying sizes can be made to accomodate specific experiments.
The authors developed these inexpensive unbreakable plexiglass plates to allow for simultaneous growth of various fungi and therefore more accurate comparison. Since these plates can be stored and handled easily, most of the disadvantages of using van Tieghem´s hanging drop culture method have been eliminated.
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